Articles EAD6


Wittmann C. and Portais JC. Metabolic Flux Analysis. In : Metabolomics in practice : successful strategies to generate and analyze metabolic data. Lämmerhofer & Weckwerth Eds., WILEY, Weinheim, Germany. ISBN: 978-3-527-33089-8, 2013


Peyraud R, Kiefer P, Christen P, Portais JC, Vorholt JA. Co-consumption of methanol and succinate by Methylobacterium extorquens AM1. PLoS One. 2012;7(11):e48271.

Giraudeau P, Cahoreau E, Massou S, Pathan M, Portais JC, Akoka S. UFJCOSY: a fast 3D NMR method for measuring isotopic enrichments in complex samples. Chemphyschem. 2012 13(13):3098-101.

Martzolff A, Cahoreau E, Cogne G, Peyriga L, Portais JC, Dechandol E, Le Grand F, Massou S, Gonçalves O, Pruvost J, Legrand J. Photobioreactor design for isotopic non-stationary 13C-metabolic flux analysis (INST 13C-MFA) under photoautotrophic conditions. Biotechnol Bioeng. 2012 109(12):3030-40.

Cahoreau E, Peyriga L, Hubert J, Bringaud F, Massou S, Portais JC. Isotopic profiling of ¹³C-labeled biological samples by two-dimensional heteronuclear J-resolved nuclear magnetic resonance spectroscopy. Anal Biochem. 2012 427(2):158-63.

Millerioux Y, Morand P, Biran M, Mazet M, Moreau P, Wargnies M, Ebikeme C, Deramchia K, Gales L, Portais JC, Boshart M, Franconi JM, Bringaud F. ATP synthesis-coupled and -uncoupled acetate production from acetyl-CoA by mitochondrial acetate:succinate CoA-transferase and acetyl-CoA thioesterase in Trypanosoma. J Biol Chem. 2012 287(21):17186-97. Article Plate-forme

Millard P, Letisse F, Sokol S, Portais JC. IsoCor: correcting MS data in isotope labeling experiments. Bioinformatics. 2012 28(9):1294-6.

Sokol S, Millard P, Portais JC. influx_s: increasing numerical stability and precision for metabolic flux analysis in isotope labelling experiments. Bioinformatics. 2012 28(5):687-93.

Schneider K, Peyraud R, Kiefer P, Christen P, Delmotte N, Massou S, Portais JC, Vorholt JA. The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate. J Biol Chem. 2012 287(1):757-66.



Heux S, Philippe B, Portais JC. High-throughput workflow for monitoring and mining bioprocess data: Application to infer E. coli physiological response to perturbations. Appl Environ Microbiol. 2011 77:7040-9

Peyraud R, Schneider K, Kiefer P, Massou S, Vorholt JA, Portais JC. Genome-scale reconstruction and system level investigation of the metabolic network of Methylobacterium extorquens AM1. BMC Syst Biol. 2011 5:189.

Enjalbert B, Jourdan F, Portais JC. Intuitive visualization and analysis of multi-omics data and application to Escherichia coli carbon metabolism. PLoS One. 2011;6: e21318.

Stoffel SA, Alibu VP, Hubert J, Ebikeme C, Portais JC, Bringaud F, Schweingruber ME, Barrett MP. Transketolase in Trypanosoma brucei. Mol Biochem Parasitol. 2011 179:1-7. Article Plate-forme

Hazard D, Fernandez X, Pinguet J, Chambon C, Letisse F, Portais JC, Wadih-Moussa Z, Rémignon H, Molette C. Functional genomics of the muscle response to restraint and transport in chickens. J Anim Sci. 2011 89:2717-30. Article Plate-forme

Laparre J, Balzergue C, Rochange S, Ludwiczak P, Letisse F, Portais JC, Bécard G, Puech-Pages V. Metabolite profiling of pea roots in response to phosphate availability. Plant Signal Behav. 2011 6(6).

Giraudeau P, Massou S, Robin Y, Cahoreau E, Portais JC, Akoka S. Ultrafast quantitative 2D NMR: an efficient tool for the measurement of specific isotopic enrichments in complex biological mixtures. Anal Chem. 2011 83:3112-9.

Irague R, Massou S, Moulis C, Saurel O, Milon A, Monsan P, Remaud-Siméon M, Portais JC, Potocki-Véronèse G. NMR-based structural glycomics for high-throughput screening of carbohydrate-active enzyme specificity. Anal Chem. 2011 83:1202-6.


Ebikeme C, Hubert J, Biran M, Gouspillou G, Morand P, Plazolles N, Guegan F, Diolez P, Franconi JM, Portais JC, Bringaud F. Ablation of succinate production from glucose metabolism in the procyclic trypanosomes induces metabolic switches  to the Gly3p/DHAP shuttle and to proline metabolism. J Biol Chem.  J Biol Chem. 2010 285:32312-24. Article Plate-forme

Walther T, Novo M, Rössger K, Létisse F, Loret MO, Portais JC, François JM. Control of ATP homeostasis during the respiro-fermentative transition in yeast. Mol Syst Biol. 2010 6:344.

El Rammouz R, Létisse F, Durand S, Portais JC, Moussa ZW, Fernandez X. Analysis of skeletal muscle metabolome: evaluation of extraction methods for targeted metabolite quantification using liquid chromatography tandem mass spectrometry. Anal Biochem. 2010 398:169-77. Article Plate-forme

Brison Y, Fabre E, Moulis C, Portais JC, Monsan P, Remaud-Siméon M. Synthesis of dextrans with controlled amounts of alpha-1,2 linkages using the transglucosidase GBD-CD2. Appl Microbiol Biotechnol. 2010 86:545-54.


      JC Rodríguez-Prados, P de Atauri, J Maury, F Ortega, JC Portais, C Chassagnole, L Acerenza, ND Lindley & M Cascante (2009) In silico strategy to rationally engineer metabolite production: a case study for threonine in E coli. Biotech. Bioeng., 103:609-620 (IF =3.037)

      Peyraud R., Kiefer P., Christen P., Massou S.,  Portais JC., Vorholt J.A. Demonstration of the ethyl-malonyl-CoA pathway using 13C-metabolomics. Proc. Nat. Acad. Sci. USA. 2009, 106 : 4846-51.

      Brito JA, Teruel H, Massou S, Gomez M. (95)Mo NMR: a useful tool for structural studies in solution. Magn Reson Chem. 2009 47:573-7



      Gomez-Roldan V., Fermas S., Brewer P.B., Puech-Pagès V., Dun E.A., Pillot JP., Letisse F., Radoslava Matusova R., Danoun S., Portais JC, Bouwmeester H, Bécard G, Beveridge C.A., Rameau C. and Rochange S.F. Strigolactone inhibition of shoot branching. Nature, 2008, 455, 189 – 194.

      Kiefer P, Portais JC & Julia Vorholt. "Quantitative metabolome analysis using liquid chromatography high resolution mass spectrometry. Analytical Biochemistry, 2008, 382:94-100

      Nicolas C., Becker J., Sanchou L., Letisse F., Wittmann C., Portais JC., and Massou S. Measurement of isotopic enrichments in 13C-labelled molecules by 1D selective Zero-Quantum Filtered TOSCY NMR experiments. Comptes-Rendus Chimie. 2008, 11 : 480-485

      McHeik A, Katir N, Castel A, Gornitzka H, Massou S, Riviere P, Hamieh T. 2008. Germylenes and Stannylenes with Chelating Anilido-Imine Ligands: Syntheses, Structures and Reactivity. European Journal Of Inorganic Chemistry 2008, 34: 5397-5403.

      Brito JA, Teruel H, Muller G, Massou S, Gomez M. Molybdenum(VI)-catalysed olefin epoxidation: Structure and reactivity study. Inorganica Chimica Acta 2008, 361:2740-2746.

      Favier I, Massou S, Teuma E, Philippot K, Chaudret B, Gomez M. 2008. A new and specific mode of stabilization of metallic nanoparticles. Chemical Communications (28):3296-3298.



      10. Chistoserdova L., Crowther G., Vorholt J.A., Skovran E., Portais JC, and Lidstrom M.E. Identification of a fourth formate dehydrogenase in Methylobacterium extorquens AM1 and confirmation of the essential role of formate oxidation in methylotrophy. J. Bact. 2007, 189 : 9076-81.

      11. Van der Werf M.J., Takors R., Smedsgaard J., Nielsen J., Ferenci T., Portais JC., Wittmann C., Hooks M., Tomassini A., Stephanopolous G., Oldiges M., Fostel J., and Sauer U. Standard Reporting Requirements for Biological Samples in Metabolomics Experiments: Microbial and in vitro biology experiments. Metabolomics, 2007, 3: 189–194

      12. Nicolas C., Kiefer P., Letisse F., Krömer J., Massou S., Soucaille P., Wittmann C., Lindley N., and Portais JC. Response of the central metabolism of Escherichia coli to modified expression of the gene encoding the glucose-6-phosphate dehydrogenase. FEBS Letters, 2007, 581: 3771-3776

      13. Troufflard S., Roscher A., Thomasset B., Barbotin JN., Rawsthorne S., and Portais JC. In vivo 13C-NMR determines metabolic fluxes and steady-state in linseed embryos. Phytochemistry, 2007, 68: 2341-2350

      14. Massou S., Nicolas C., Letisse F., and Portais JC. Application of 2D-TOCSY NMR to the measurement of specific 13C-enrichments in complex mixtures of 13C-labeled metabolites. Metabolic Engineering, 2007 9: 252-257.

      15. Massou S., Nicolas C., Letisse F., and Portais JC. NMR-based fluxomics: Quantitative 2D-NMR Methods for Isotopomer Analysis. Phytochemistry. 2007, 68: 2330-2340.

      16. Bolten C.J., Kiefer P., Letisse F., Portais JC., and Wittmann C.. Towards appropriate sampling for metabolome analysis of microorganisms. Analytical Chemistry, 2007; 79:3843-9.

      17. Kiefer P, Nicolas C, Letisse F, Portais JC. Determination of carbon labeling distribution of intracellular metabolites from single fragment ions by ion chromatography tandem mass spectrometry. Analytical Biochem. 2007 360:182-8.


      18. Besserer A, Puech-Pages V, Kiefer P, Gomez-Roldan V, Jauneau A, Roy S, Portais JC, Roux C, Becard G, Sejalon-Delmas N. Strigolactones stimulate arbuscular mycorrhizal fungi by activating mitochondria. PLoS Biol. 2006, 4:e226

      19. Chistoserdova, M. Laukel, J.-C. Portais, J.A. Vorholt, and M.E. Lidstrom. Multiplicity of formate dehydrogenase enzymes in the facultative methylotroph Methylobacterium extorquens AM1 and their dispensability for growth on methanol. J. Bacteriol. 2004, 186: 22-28.